Research serial dilution planner
Calculate transfer and diluent volumes for a constant-factor laboratory dilution series.
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Use positive concentration and volume, factor>1 and 1–30 whole steps. Concentration and volume units are chosen by you and must remain consistent. Research bench planning only, never medication dosing.
The starting values are hypothetical. Results update as you edit. Use aggregate or synthetic inputs only.
Calculation results
- Transfer per step
- 0.5
- Diluent per tube
- 0.5
- Concentrations by step
- 50.000000, 25.000000, 12.500000, 6.2500000, 3.1250000
- Volume left after transferring to next tube
- 0.5
- Volume left in last tube
- 1
- Total diluent
- 2.5
- Restriction
- Research solutions only; not clinical preparation or dosing guidance.
How to interpret the output
Each step mixes final volume/factor of the preceding solution with the remaining diluent. The concentration divides by factor at each step. The previous tube loses transfer volume, so its remaining volume is lower than the prepared volume. Pipette minimums, mixing losses, stability and material compatibility are not evaluated.
Method referenceSource checked 2026-11-06. Read the reproducible calculator validation report.